WD 40G Acronis clone to WD 250G HDD

I have made a clone of my WD 40 gig hdd to a new WD250 gig hdd. Works fine, booted from it, using it now. I’m having a problem adding my backup drive back into the system though. Originally I had the WD 40 gig (C:/) and a Seagate 500 gig (D:/) running perfectly in this system. Now when I hook the 500 gig back into the system my computer won’t boot at all. Jumpers all set as before, even tried different configurations of jumpers, but no luck. I get an error when starting up, only choices are F1 to continue or DEL to enter setup. Delete takes me to bios, bios see’s both drives.

If I put the 40 gig in as a slave, computer starts, see’s both drives, I can access both drives, everything fine

Original 40 gig and 500 gig as slave boots.

New 250 gig and 40 gig as slave boots.

New 250 by itself boots.

Old 40 gig by itself boots.

Any idea whats going on here?

Anyone help me get this working?