Did you try what was suggested above? Access the Twonky administration page and perform a Clear Cache then Rescan Content Folders. If that doesn’t work try to restart the Twonky server by selecting Restart Server.
There is much information on Twonky in the unofficial Twonky FAQ, in particular a section called How do I clear Twonky’s database and start again? that may help. That section is in the following post:
