Dedicated Hard Drive

Hi, new to this technology but i have purchased a EX4100 and would like to know if you can provide each hard drive a dedicated location i.e. Bay 1 being G: drive Bay 2 being H: drive etc within finder on mac and computer on windows.
reason being, run a small business and want to keep employees access only to bay 1 and home/family stuff to bay 2 as example.
thanks for being understanding.